Fig. 2: Depletion of SRSF3 induced apoptosis, senescence, and an increase in p21 ^cip1/waf1 was independent of p53.

A HCT116 p53−/− and p53+/+ cells were transfected with siControl or siSRSF3 #1, #2 and subjected to cell cycle analysis by flow cytometry. B Silencing of SRSF3 in HCT116 p53−/− or p53+/+ cells, which were stained with annexin V and PI, and subjected to flow cytometry. C The siControl or siSRSF3 were transfected into HCT116 p53−/− or p53+/+ cells for 72 h and analyzed by Western blot analysis for the indicated antibodies. D After transfection of p53−/− or p53+/+ HCT116 cells with empty vector or p53β plasmid DNA for 36 h, cells were stained with SA-β Galactosidase. Scale bar, 100 μm. E Cell lysates were prepared from the HCT116 p53−/− or p53+/+ cells after transfection with p53β plasmid DNA for 36 h. The level of p53 α, β, p21^cip1/waf1, and β-actin were measured by western blot analysis. F The siControl or siSRSF3 were transfected into HCT116 p53−/− or p53+/+ cells for 72 h and analyzed by Western blot analysis for the indicated antibodies. G The levels of p21 mRNA in HCT116 p53−/− or p53+/+ cells transfected with either control or SRSF3 siRNA. Data are shown as mean ± SD. ***P < 0.001, two-tailed Student’s t-test. H SA-β-Gal staining in HCT116 p53−/− or p53+/+ cells after 72 h of siControl or siSRSF3 treatment, Scale bar, 100 μm.