Fig. 3: SRSF3 regulates translation of p21cip1/waf1 mRNA in cytoplasm.
From: Splicing factor SRSF3 represses translation of p21cip1/waf1 mRNA
A, B After treatment with siControl or siSRSF3 for 72 h, SW480 cells were untreated or treated with 10 μM cycloheximide (CHX) for 24 h. Data are shown as mean ± SD. ns, not significant; ***P < 0.001, **p < 0.01, two-tailed Student’s t-test. C Cells were transfected with control and SRSF3 siRNAs and separated by polysome fraction. RNA samples from each fraction were subjected to RT-PCR analysis to detect CDKN1A (p21cip1/waf1). GAPDH was used as control. D Western blot analysis was performed with whole cell extract from (C). Quantification of RT-PCR analysis from (C) was performed to observe relative abundance for Cdkn1a and GAPDH in each fraction (E). Free mRNP (Fraction 1–3), monosome (Fraction 4–8), light polysome (Fraction 9–11), and heavy polysome (Fraction 12–16) (F). Data are shown as mean ± SD. ***P < 0.001, two-tailed Student’s t-test.
