Fig. 4: FGF2 secreted by MSCs bound to FGFR2 on T-ALL cells and activated PI3K signalling pathway. | Cell Death & Disease

Fig. 4: FGF2 secreted by MSCs bound to FGFR2 on T-ALL cells and activated PI3K signalling pathway.

From: Blockade of FGF2/FGFR2 partially overcomes bone marrow mesenchymal stromal cells mediated progression of T-cell acute lymphoblastic leukaemia

Fig. 4

A RNA-seq cluster diagram of primary murine MSCs isolated from leukaemia and control mice (Red colour meant the genes were up-regulated and green colour meant the genes were down-regulated). B Heat map of primary murine T-ALL cells co-cultured with MSCs from leukaemia or control mice for 16 h (red colour meant the genes were up-regulated and green colour meant the genes were down-regulated). C Schematic representation of analysis strategies integrating expression profiles obtained from two RNA-seqs. D Realtime PCR was used to validate the expression pattern of selected six cytokine genes in MSCs and found that FGF2 was significantly up-regulated in MSCs from leukaemia mice. E Quantitative analysis of four FGF receptors showed that FGFR2 was significantly up-regulated in murine T-ALL cells cultured with leukaemia derived MSCs. F Dose response curve of BGJ398 with different concentration revealed that IC50 was 1 μM. G The knockdown efficiency of FGF2 in MSCs after sh-RNA transfection. H The phosphorylation of key proteins of PI3K/AKT/mTOR signalling pathway in T-ALL cells after addition of BGJ398 or FGF2 knockdown were analysed by Western blot. (n ≥ 3, *P < 0.05, **P < 0.01, ***P < 0.005).

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