Fig. 5: The growth of T-ALL cells was suppressed after FGF2/FGFR2 blockade in vitro co-culture system.

A Growth curve of Jurkat, TALL-1 and primary murine T-ALL cells co-cultured with MSCs from leukaemic mice supplemented with BGJ398. Vertical legend meant the ratio of surviving leukaemia cells to the initial number of leukaemia cells. B Jurkat, TALL-1, or primary murine T-ALL cells were co-cultured with leukaemic mice derived MSCs. Growth curve showed that the growth of T-ALL cells were inhibited after FGF2 knockdown in MSCs. C BGJ398 (1 μM) was added to the co-culture system and 12 h later, the apoptosis rates of primary murine T-ALL cells (upper), Jurkat (lower) were increased. D Percentage of T-ALL cells in S phase was decreased after 12 h co-culture with BGJ398. E The apoptosis rates of primary murine T-ALL cells (upper), Jurkat (lower) were increased after co-culture with FGF2 knockdown MSCs for 12 h. F Percentage of T-ALL cells in S phase was decreased after co-culture with FGF2 knockdown MSCs for 12 h. G PCR results revealed that mRNA expression of cell cycle and apoptosis associated genes in T-ALL cells changed after addition of BGJ398 to the co-culture system. H Expression changes of cell cycle and apoptosis associated mRNA in T-ALL cells after co-culture with FGF2 knockdown MSCs. I, J Western blot results also revealed that P21, P27 and BAX were up-regulated and CDK2 was down-regulated after FGF2 and FGFR2 blockade. (n ≥ 3, *P < 0.05, **P < 0.01, ***P < 0.005).