Fig. 3: RHBDL2 promotes proliferation and mobility of PC cells by activating the Notch signaling pathway. | Cell Death & Disease

Fig. 3: RHBDL2 promotes proliferation and mobility of PC cells by activating the Notch signaling pathway.

From: RHBDL2 promotes the proliferation, migration, and invasion of pancreatic cancer by stabilizing the N1ICD via the OTUD7B and activating the Notch signaling pathway

Fig. 3

A The result of RNA-seq analysis. B, C qRT-PCR and Western blot analyses of RHBDL2, HES1, HEY1, ZEB1, MMP9, SNAIL1, and TWIST1 in the indicated PC cells. D Dual Luciferase Reporter Assay. CCK-8 (E), colony formation (F), and EdU (scale bar: 50 μm) (G) assays of the PC cells incubated with/without IMR-1 (the inhibitor of Notch signaling pathway). Wound healing (scale bar: 100 μm) (H) and Transwell (scale bar: 50 μm) (I) assays of the indicated PC cells incubated with/without IMR-1. J, K qRT-PCR and Western blot analyses of HES1, HEY1, ZEB1, MMP9, SNAIL1, and TWIST1 in the indicated PC cells incubated with/without IMR-1. (L) Dual Luciferase Reporter Assay of the indicated PC cells incubated with/without IMR-1. *P < 0.05.

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