Fig. 5: RHBDL2 inhibits the degradation of N1ICD via ubiquitin-proteasome pathway. | Cell Death & Disease

Fig. 5: RHBDL2 inhibits the degradation of N1ICD via ubiquitin-proteasome pathway.

From: RHBDL2 promotes the proliferation, migration, and invasion of pancreatic cancer by stabilizing the N1ICD via the OTUD7B and activating the Notch signaling pathway

Fig. 5

A N1ICD and RHBDL2 levels were confirmed by Western blot analysis in the indicated PC cells treated with 40 μg/ml of cycloheximide (CHX). The indicated PC cells were treated with chloroquine (B), NH4Cl (C), 3-MA (D), or MG132 (E), respectively. Then, the level of N1ICD was detected by Western blot analysis. F The PC cells were treated with 15 μM of MG132 for 6 h prior to harvest. Cell lysate was immunoprecipitated with Myc-tag antibody and immunoblotted as indicated.

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