Fig. 2: Yeast Set2 contributes to the observed upregulation of Atg gene expression under nutrient-deprivation conditions.
From: SETD2 transcriptional control of ATG14L/S isoforms regulates autophagosome–lysosome fusion
a–c Wild-type (WT) and (b, c) set2Δ yeast strains were grown in rich medium conditions until mid-log phase. Cells were shifted to a nitrogen-starvation medium (–N) for 2 h or left in nutrient-rich conditions (+N). Thereafter, RT-qPCR analyses were performed to assess the mRNA expression levels of Atg5, Atg7, Atg8, Atg12, Atg14, and Atg16 genes. a Induction of expression for all tested Atg genes was observed upon starvation in the WT yeast strain. b Under nutrient-rich conditions, Set2 gene depletion in yeast (set2Δ) had, except for Atg16, no significant effect on the expression of the Atg genes tested. c Starved set2Δ yeast showed significantly lower expression for most of the tested Atg genes, as compared to starved WT yeast. Bars display the mean of three independent experiments (n = 3), error bars represent SEM; *P < 0.05; **P < 0.01; ***P < 0.001; ns: non-significant.
