Fig. 1: High expression of CD38 causes oxidative degradation of DHFR.
From: Oxidative degradation of dihydrofolate reductase increases CD38-mediated ferroptosis susceptibility
A Western blot analysis confirmed the overexpression of CD38 in A549-CD38 cells compared with A549-Plvx cells. B Relative ROS levels in A549-Plvx and A549-CD38 cells (n = 3). C and D NAD+ and NADP+ levels in A549-Plvx and A549-CD38 cells were determined by peak areas from the metabolomics analysis (n = 3). E Relative ROS levels of A549 cells treated with or without 100 nM FK866 for 12 h (n = 3). F A549 cells were treated with or without 100 nM FK866 for 12 h. Protein levels of DHFR and Actin (loading control) were analyzed by western blot. Graphs represent the quantification of the blots (n = 3). G The relative transcription levels of DHFR in untreated and 100 nM FK866-treated A549 cells (n = 3). H The relative ROS levels in A549-CD38 cells treated with or without 1 mM NMN for 12 h (n = 3). I A549-Plvx and A549-CD38 cells were treated with or without 1 mM NMN. Protein levels of DHFR and Actin (loading control) were analyzed by western blot. Graphs represent the quantification of the blots (n = 3). J The relative transcription levels of DHFR in A549-Plvx and A549-CD38 cells treated with or without 1 mM NMN (n = 3). Data were shown as mean ± SD and analyzed by Student’s t-test or one-way ANOVA test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
