Fig. 2: The oxidative degradation of DHFR is mediated by the autophagy and non-canonical proteasome pathways.
From: Oxidative degradation of dihydrofolate reductase increases CD38-mediated ferroptosis susceptibility
A Relative ROS levels were measured in A549 cells treated with different concentrations of H2O2 (0, 0.05, 0.1, 0.5, 1, 2 mM) for 12 h (n = 3). B A549 cells were treated with different concentrations of H2O2 (0, 0.05, 0.1, 0.5, 1, 2 mM) for 12 h. Protein levels of DHFR and Actin (loading control) were analyzed by western blot. Graphs represent the quantification of the blots (n = 3). C The transcription levels of DHFR in A549 cells treated with different concentrations of H2O2 (0, 0.05, 0.1, 0.5, 1, 2 mM) for 12 h (n = 3). D Western blot images of DHFR and Actin (loading control) in untreated and 1 mM H2O2-treated A549 cells pre-treated with 6 mM NAc for 6 h or not. Graphs represent the quantification of the blots (n = 3). E Western blot images of DHFR and Actin (loading control) in untreated and 1 mM H2O2-treated A549 cells co-treated with 100 nM Baf-A1 or not. Graphs represent the quantification of the blots (n = 3). F Western blot images of DHFR and Actin (loading control) in untreated and 1 mM H2O2-treated A549 cells pre-treated with 10 μM MG132 for 4 h or not. Graphs represent the quantification of the blots (n = 3). G Western blot images of DHFR and Actin (loading control) in DMSO, NAc (6 mM, 6 h), Baf-A1 (100 nM, 6 h), and MG132 (10 μM, 4 h)-treated A549-CD38 cells. Graphs represent the quantification of the blots (n = 3). Data were shown as mean ± SD and analyzed by one-way ANOVA test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
