Fig. 3: The sulfonation of Cys7 in DHFR induces its oxidative degradation.
From: Oxidative degradation of dihydrofolate reductase increases CD38-mediated ferroptosis susceptibility
A The mass spectrometry spectrum of the peptide containing sulfonated Cys7 in DHFR, with the inset illustrating the ion intensity of the peptide from untreated (Control) and H2O2 treated A549-DHFR cells with pre-treatment of MG132 (H2O2 + MG132). B Western blot images of DHFR, Flag-tagged DHFR, and Actin in A549-Plvx, A549-DHFR-C7A, and A549-DHFR-C7D cells. C Western blot images of DHFR-C7D protein in DMSO, Baf-A1 (100 nM, 6 h) treated, and MG132 (10 μM, 4 h)-treated A549-C7D cells. D and E Western blot analysis of DHFR and Flag-tagged DHFR proteins from untreated and H2O2-treated A549-DHFR and A549-DHFR-C7A cells, respectively. Graphs represent the quantification of the blots (n = 3). Data were shown as mean ± SD and analyzed by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
