Fig. 1: LINC02551 is regulated by ALKBH5 in an m6A -dependent manner. | Cell Death & Disease

Fig. 1: LINC02551 is regulated by ALKBH5 in an m6A -dependent manner.

From: ALKBH5-mediated m6A modification of lincRNA LINC02551 enhances the stability of DDX24 to promote hepatocellular carcinoma growth and metastasis

Fig. 1

a Volcano plot of the analysis of the RNA-seq of HLF cells with and without ALKBH5 overexpression. Red dots indicate the upregulated genes; green dots indicate the downregulated genes. b qRT-PCR analysis of LINC02551 expression in 97H and HLF cells with knockdown and overexpression of ALKBH5. c MeRIP results showing LINC02551 in HLF cells with ALKBH5 overexpression. d Schematic diagram showing ALKBH5-wt and ALKBH5-mut. e qRT-PCR analysis of LINC02551 expression in 97H cells transfected with ALKBH5-wt (left panel) and ALKBH5-mut (right panel) via dose gradient. f Schematic diagram of LINC02551 putative m6A modification sites. g Relative luciferase activity in HLF cells cotransfected with psiCHECK2 containing the LINC02551 sequence (wt and mutated m6A sites) and an ALKBH5 plasmid. h The degradation rate of LINC02551 in 97H cells with ALKBH5 overexpressed (right panel) or knocked down (right panel) and treated with actinomycin D over time. i Relative LINC02551 level in 97H and HLF cells transfected with siIGF2BP1. j Abundance of IGF2BP1 targeting LINC02551 in HLF cells transfected with ALKBH5-wt or ALKBH5-mut. k The MS2 RNA pull-down results of LINC02551-wt and LINC02551-m1. GAPDH was the negative control and Flag was the positive control. l Schematic diagram showing IGF2BP1-wt and IGF2BP1-mut. m Relative LINC02551 level in 97H and HLF cells transfected with IGF2BP1-wt or IGF2BP1-mut. n Relative LINC02551 level in 97H and HLF cells transfected with siALKBH5 alone or together with siIGF2BP1. o MeRIP results showing LINC02551-wt or LINC02551-mut in HLF cells with ALKBH5-wt or ALKBH5-mut overexpression. p Representative IHC/ISH staining results in paraffin-embedded HCC samples from Tongji Hospital (left panel). The significance of LINC02551 and ALKBH5 expression is indicated in the right panel. And this analysis was performed using F-test. The remaining statistical analysis was performed using Student’s t-tests. Data are mean ± SEM. *P < 0.05, **P < 0.001, ***P < 0.0001.

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