Fig. 4: LINC02551 decreased the ubiquitination-induced degradation of DDX24. | Cell Death & Disease

Fig. 4: LINC02551 decreased the ubiquitination-induced degradation of DDX24.

From: ALKBH5-mediated m6A modification of lincRNA LINC02551 enhances the stability of DDX24 to promote hepatocellular carcinoma growth and metastasis

Fig. 4

a Representative peptide sequences of DDX24. b The RNA pull-down assay of the combination between LINC02551 and DDX24. c The RIP results of the interaction between DDX24 and LINC02551. d IF assay coupled with FISH was performed. e The RNA pull-down assay showing the combination between truncation LINC02551 mutants and DDX24. f, g Relative DDX24 RNA (f) and protein levels (g) in HLF and 97H cells transfected with LINC02551 in dose gradients. h, i WB and statistical analysis of DDX24 expression in HLF and 97H cells after CHX (cycloheximide, 40 μg/ml) treatment. j HLF and 97H cells treated with DMSO, proteasome inhibitor MG132 (20 μM) or the lysosome inhibitor CQ (Chloroquine, 100 μM) for 4 h. The endogenous DDX24 level was detected by WB. Statistical analysis was performed using Student’s t-tests. Data are mean ± SEM. *P < 0.05, **P < 0.001, ***P < 0.0001, ns means no significance.

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