Fig. 3: PRMT3 deficiency induces cell cycle arrest and cell death.
From: PRMT3 drives glioblastoma progression by enhancing HIF1A and glycolytic metabolism
A, B Flow cytometry cell cycle analysis (A) and quantification (B) of shCtrl and shPRMT3-1 (PRMT3 KD) GSC20 cell cultures. Cells were transduced with control or shPRMT3-1 lenti-virus after 48 h. C, D Representative flow cytometry analysis of cell apoptosis (left) and quantification (right). Cells were transduced with control or shPRMT3 lenti-virus after 2 days (C) or 6 days (D). E-G Representative western blots for cleaved-caspase 3 (CC3) and PRMT3 in GSC20 (E), GSC627 (F) and U251 (G) transduced with lenti-shCtrl and lenti-shPRMT3 for 3 days. ACTIN served as a loading control. Data are presented as means ± SEM; n = 3 independent experiments; **p < 0.01; ***p < 0.001; two-tailed unpaired Student t-test (in B) or one-way ANOVA with multiple comparison test (in C, D). N.S., not significant.
