Fig. 7: PRMT3 binds and regulates the expression and function of HIF1A.
From: PRMT3 drives glioblastoma progression by enhancing HIF1A and glycolytic metabolism
A Plot showing the correlation between the mRNA levels of PRMT3 and HIF1A in the CCGA database. r, correlation score; p value, statistical significance of the correlation score. B Dual luciferase assay for HIF1A reporter activity. Data are presented as means ± SEM; n = 3 independent experiments; ***p < 0.001; two-tailed unpaired Student t-test. C Representative western blots for HIF1A and PRMT3 in U251Ctrl cells and PRMT3-OE cells under hypoxia. ACTIN served as a loading control. D, E Representative western blots for HIF1A and PRMT3 in U251 (D) and GSC262 (E) Ctrl cells and PRMT3-KD cells under hypoxia. ACTIN served as a loading control. F The half-life of endogenous HIF-1α detected by western blot after treatment with cycloheximide (CHX) under hypoxia. G CO-IP assay for the interaction of endogenous PRMT3 and HIF1A in the GSC262 cell line under hypoxia. H Representative western blots for poly-ubiquitin levels of HIF1A in 293 T PRMT3-KD (left) and PRMT3 overexpression (OE) (right) cells. ACTIN served as a loading control. I, J qRT-PCR analysis of the indicated genes in Ctrl and PRMT3-KD GSC20 (I) and GSC295 (J) cell lines. Data are presented as means ± SEM; n = 3 independent experiments; *p < 0.05, **p < 0.01; ***p < 0.001; one-way ANOVA with multiple comparisons test. K qRT-PCR analysis of the indicated genes in Ctrl and PRMT3-OE GSC295 cells. Data are presented as means ± SEM; n = 3 independent experiments; ***p < 0.001; two-tailed unpaired Student t-test.
