Fig. 7: IL-18 knockdown attenuated necroptosis, transdifferentiation, and fibrosis in HK-2 cells.
From: IL-18 deficiency ameliorates the progression from AKI to CKD
A The mRNA expression of IL-18 in HK-2 cells with hTGF-β stimulation by qPCR. B The protein expression of IL-18 HK-2 cells treated with hTGF-β and additional transfected with IL-18 siRNA and its scramble analyzed by Western blotting. C The protein expression of necroptosis indicated by RIPK1 and RIPK3 at 12 h after the transfection of IL-18 siRNA on Western blotting. D The expression of transdifferentiation marked by TGF- β1, vimentin, and E-cadherin at 24 h after the transfection of IL-18 siRNA on Western blotting. E The expression of fibrotic COL-1 at 48 h after the transfection of IL-18 siRNA on Western blotting (n = 3, #p < 0.05, other groups vs. NC; *p < 0.05, IL-18 siRNA vs. IL-18 scramble).
