Fig. 5: LJ2 inhibits cell death induced by microtubule destabilization and hydroxamic acid-based deacetylase (HDAC) inhibition.

a Effect of the irreversible Caspase-2 inhibitors LJ2 against vincristine-induced cell death. HeLa cells were pretreated for 1 h with the indicated doses of LJ2, Q-VD-OPh, or Q-LETD-OPh, then treated with vincristine (VCR) as in Fig. 2, then incubated with propidium iodide at 2 μg/mL for 10 min and analyzed by flow cytometry. Histogram data are mean ± SD (n = 3, *p value < 0.01). b Effect of Caspases-inhibitors against Panobistat (LBH589)-induced cell death. Molt4 lymphoid human cells where were pretreated for 1 h with 30 µM of LJ2, Q-VD-OPh, or Belnacasan (VX-765), then treated for 24 h with 25 nM of LBH589, then incubated with propidium iodide as in (a) and analyzed by flow cytometry. Histogram data are mean ± SD (n = 4, ***p value < 0.001). c Dose–response of LJ2-mediated cell death inhibition. Molt4 cells were treated as in (b) with the indicated doses of LJ2 or Q-VD-OPh and analyzed by flow cytometry. Histogram data are mean ± SD (n = 3, **p value < 0.01).