Fig. 6: EV transfer of miR-769-5p promotes MMT reversal. | Cell Death & Disease

Fig. 6: EV transfer of miR-769-5p promotes MMT reversal.

From: Restoration of WT1/miR-769-5p axis by HDAC1 inhibition promotes MMT reversal in mesenchymal-like mesothelial cells

Fig. 6

A RT-PCR showing expression of miR769-5p in whole cell extract (WCE) (left) or the EV fraction (right) upon miR769-5p ectopic expression in primary MCs from PD patients. miR-16 was used for normalization. Bars represent the mean ± SEM of duplicate determinations in three independent experiments B RT-PCR showing expression of TGFβ1, PAI-1, SMAD2 and SMAD3 mRNA from total RNA of recipient MCs after treatment with EVs (10 μg) from MCs transfected with control or mimic miR769-5p. Bars represent the mean ± SEM of duplicate determinations in six independent experiments. C WB showing the expression of Smad2, Smad3, TGFBRI and PAI-1 in MCs from PD patients after treatment with EVs expressing miR769-5p (generated as Fig. 6A). Hsp90 was used as a loading control. Data are representative of three independent experiments.

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