Fig. 2: Identification of PRDM15 as a radioresistant gene in CRC. | Cell Death & Disease

Fig. 2: Identification of PRDM15 as a radioresistant gene in CRC.

From: PRDM15 interacts with DNA-PK-Ku complex to promote radioresistance in rectal cancer by facilitating DNA damage repair

Fig. 2

A, B The level of PRDM15 expression shown by Western Blot in HCT116 infected with PRDM15-specific shRNA or PRDM15 overexpression plasmids. C Cell proliferation of HCT116 in different groups after 8 Gy IR determined by CCK-8 assay. Cells transfected with shNC served as controls. Error bars represent the SD of the mean of three independent experiments. Two tailed Student’s t test was applied in NC and KD groups, respectively, after analyzing with ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus untreated control. D–F Cell survival and its representative images of HCT116 in different groups after 0, 2, 4, 6 Gy IR. Colony analysis was performed at 12 days after IR. Cells transfected with shNC served as controls. Error bars represent the SD of the mean of three independent experiments, two tailed Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus untreated control. G, H The apoptosis of HCT116 after 8 Gy IR at 24 h in different groups was analyzed by flow cytometry. AV means Annexin V. Cells transfected with shNC served as controls. Error bars represent the SD of the mean of three independent experiments. One-way ANOVA was performed in the three groups, *P < 0.05, **P < 0.01. I The cell cycle of HCT116 after 8 Gy IR at 0,4,8,12,24 h in different groups was analyzed by flow cytometry, n = 6 for each group per time point. Values are given as mean ± SD, two-tailed Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus untreated control.

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