Fig. 3: PRDM15 affects the process of radiation-induced DNA damage response. | Cell Death & Disease

Fig. 3: PRDM15 affects the process of radiation-induced DNA damage response.

From: PRDM15 interacts with DNA-PK-Ku complex to promote radioresistance in rectal cancer by facilitating DNA damage repair

Fig. 3

A Representative images from the comet assay of PRDM15-KD and NC cells at 8 h after 8 Gy IR. The tail DNA percentage (B) and tail moment (C) were quantified with CASP 1.2.3b2 software in PRDM15-KD and NC cells. Error bars represent the SD of the mean of three independent experiments, two tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus shNC group. D Representative images from the comet assay of PRDM15-OE and control cells at 8 h after 8 Gy IR. The tail DNA percentage (E) and tail moment (F) were quantified with CASP 1.2.3b2 software in PRDM15-OE and NC cells. Error bars represent the SD of the mean of three independent experiments, two tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus shNC group. G The γ-H2AX and 53BP1 foci in HCT116 after 8 Gy IR in different groups was analyzed by immunofluorescence staining. H, I Quantitative analysis of the number of γ-H2AX and 53BP1 foci per cell in different groups, n = 30 for each group per time point. Values are given as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus shNC group.

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