Fig. 8: N-acetylcysteine attenuates the phenotype of SMS cells. | Cell Death & Disease

Fig. 8: N-acetylcysteine attenuates the phenotype of SMS cells.

From: Retinoic acid-induced 1 gene haploinsufficiency alters lipid metabolism and causes autophagy defects in Smith-Magenis syndrome

Fig. 8

A Left panel: Representative fluorescence microscopy images of lipid droplets (LDs) colored with Oil Red O in four SMS and four CTR cells (CTR1/2/3 and sibling) treated with N-acetylcysteine (NAC, 10 μM, 24 h) (n = 5 biological replicates). Right panel: Quantification of the optical density of 10 independent wells from five biological replicates. B Trypan blue analysis of cell death in four SMS and four CTR cell lines (CTR1/2/3 and sibling) treated with NAC (10 μM, 24 h) (n = 5 biological replicates). Graphs: mean ± SEM, (B) one-way ANOVA + Newman–Keuls post hoc test, (C) unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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