Fig. 5: Knockdown of miR-3202 rescues KD-linc00976-mediated inhibitory effect on the proliferation, metastasis, and ferroptosis in CCA cells. | Cell Death & Disease

Fig. 5: Knockdown of miR-3202 rescues KD-linc00976-mediated inhibitory effect on the proliferation, metastasis, and ferroptosis in CCA cells.

From: JUND/linc00976 promotes cholangiocarcinoma progression and metastasis, inhibits ferroptosis by regulating the miR-3202/GPX4 axis

Fig. 5

a CCA cells were transfected with KD-linc00976-NC or KD-linc00976 or co-transfected with KD-linc00976 and miR-3202 inhibitors. Colony formation assays detected the cell proliferation ability of CCA cells in each group. b–f Effects of miR-3202 silencing on KD-linc00976-mediated ROS, GSH, MDA, iron progression, and GPX activities in CCA cells. j Transwell migration and Matrigel invasion assays evaluated the effect of miR-3202 silencing on KD-linc00976-mediated migration and invasion ability. Scale bar, 50 μm. h The protein expressions of ferroptosis and EMT-associated biomarkers were analyzed by western blotting and normalized to GAPDH. *P < 0.05, **P < 0.01. CCA cholangiocarcinoma, EMT epithelial–mesenchymal transition, GPx glutathione peroxidase, GSH glutathione, MDA malondialdehyde, ROS reactive oxygen species.

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