Fig. 6: linc00976 positively regulates GPX4 expression via sponging miR-3202 in CCA cells. | Cell Death & Disease

Fig. 6: linc00976 positively regulates GPX4 expression via sponging miR-3202 in CCA cells.

From: JUND/linc00976 promotes cholangiocarcinoma progression and metastasis, inhibits ferroptosis by regulating the miR-3202/GPX4 axis

Fig. 6

a Schematic illustration showing overlapping of the target mRNAs of miR-3202 predicted by TargetScan and miRPathDB. b The downregulated mRNAs were subjected to KEGG enrichment analysis. c Venn diagram showing overlapping of target mRNAs and downregulated mRNAs of miR-3202. d RT-qPCR showed the mRNA levels of six candidate genes in CCA cells transfected with KD-linc00976 or KD-linc00976-NC. e RT-qPCR showed the mRNA levels of six candidate genes in CCA cells transfected with OE-linc00976 or OE-linc00976-NC. f The putative binding sites between miR-3202 and GPX4. g The luciferase activities were detected in CCA cells after co-transfection with GPX4-WT or GPX4-MUT and miR-3202 mimics or NC mimics. h IHC images showed the co-expression of linc00976, miR-3202, and GPX4. Scale bar, 100 μm. i Volcano plots demonstrating altered metabolite levels. Metabolites associated with GSH metabolism and folate biosynthesis are significantly downregulated, while oxidized phospholipids are significantly upregulated. j KEGG enrichment analysis of differentially abundant metabolites identified in negative and positive ion modes, respectively. n.s. not significant, *P < 0.05, **P < 0.01. CCA cholangiocarcinoma, GSH glutathione, IHC immunohistochemistry, KEGG Kyoto Encyclopedia of Genes and Genomes, RT-qPCR reverse transcription-quantitative PCR.

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