Fig. 7: Gain-of-function assays confirm the involvement of linc00976/miR-3202/GPX4 axis in CCA progression. | Cell Death & Disease

Fig. 7: Gain-of-function assays confirm the involvement of linc00976/miR-3202/GPX4 axis in CCA progression.

From: JUND/linc00976 promotes cholangiocarcinoma progression and metastasis, inhibits ferroptosis by regulating the miR-3202/GPX4 axis

Fig. 7

a KD-linc00976 stable CCA cells were transfected with miR-3202 inhibitor or co-transfected with miR-3202 inhibitor and ML-210. Colony formation assays evaluated the effect of the linc00976/miR-3202/GPX4 axis on cell proliferation. b–f Effects of the linc00976/miR-3202/GPX4 axis on ROS, GSH, MDA, iron progression, and GPX activities in CCA cells. g Transwell migration and Matrigel invasion assays evaluated the effect of the linc00976/miR-3202/GPX4 axis on the migration and invasion ability of CCA cells. Scale bar, 50 μm. h The protein expressions of ferroptosis and EMT-associated were analyzed by western blotting and normalized to GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001. CCA cholangiocarcinoma, EMT epithelial–mesenchymal transition, GPx glutathione peroxidase, GSH glutathione, MDA malondialdehyde, ROS reactive oxygen species.

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