Fig. 8: JUND regulates the expression of linc00976 by binding to the JUND promoter. | Cell Death & Disease

Fig. 8: JUND regulates the expression of linc00976 by binding to the JUND promoter.

From: JUND/linc00976 promotes cholangiocarcinoma progression and metastasis, inhibits ferroptosis by regulating the miR-3202/GPX4 axis

Fig. 8

a The motif of JUND. b The binding site in the promoter of linc00976 is shown. c CCA cells were transfected with either a full‐length or truncated linc00976 promoter‐pGL3 reporter vector and further cultured, either with or without JUND plasmid. After 48 h, luciferase activity was measured using the dual‐luciferase reporter assay system. d ChIP assays with anti-JUND antibody verifying the binding between JUND and response element of the linc00976 promoter. e Co-expression relationship between linc00976 and JUND based on data from CCA tissues. f Co-expression relationship between JUND and GPX4 based on the data from our additional CCA tissues. Scale bar, 100 μm. g, h RT-qPCR and western blotting detected the effect of JUND overexpression on the mRNA and protein expression of GPX4. n.s. not significant, ***P < 0.001. CCA cholangiocarcinoma, ChIP chromatin-immunoprecipitation, RT-qPCR reverse transcription-quantitative PCR.

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