Fig. 5: TIMP2 and TIMP3 are functional targets of miR-197-3p in HUVECs. | Cell Death & Disease

Fig. 5: TIMP2 and TIMP3 are functional targets of miR-197-3p in HUVECs.

From: Cancer-derived exosomal miR-197-3p confers angiogenesis via targeting TIMP2/3 in lung adenocarcinoma metastasis

Fig. 5

A Putative binding sites between TIMP2 3’UTR and miR-197-3p. B Putative binding sites between TIMP3 3’UTR and miR-197-3p. C, D Relative luciferase activity was measured by dual luciferase reporter assay. E The miR-197-3p level in different HUVECs was determined by qRT-PCR. F The mRNA levels of TIMP2 and TIMP3 in different HUVECs were determined by qRT-PCR. G The protein levels of TIMP2 and TIMP3 in different HUVECs were determined by western blot. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page