Fig. 8: Exosomal miR-197-3p promotes lung metastasis and angiogenesis in vivo by targeting TIMP2/3.

A Photographs and quantitative analysis of pulmonary metastatic nodules. B Histopathological changes of lung tissues were assessed by H&E staining. C CD31 expression in pulmonary metastatic nodules was assessed by IHC. D The protein levels of TIMP3, VEGFR2, ERK, and p-ERK in pulmonary metastatic nodules were determined by western blot. E The protein levels of TIMP2, MMP2, and MT1-MMP in pulmonary metastatic nodules were determined by western blot. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001.