Fig. 4: TINCR regulated ATG5-mediated autophagy. | Cell Death & Disease

Fig. 4: TINCR regulated ATG5-mediated autophagy.

From: The long noncoding RNA TINCR promotes self-renewal of human liver cancer stem cells through autophagy activation

Fig. 4

A GO pathway analysis showed the involvement of autophagy in TINCR regulation. B After TINCR depletion, the level of autophagy-related proteins were detected by western blotting. C LC3 fluorescence intensity was assessed by confocal microscope. Scale bar: 10 μm. D Representative images of autophagosome detected by TEM. E ATG5 expression in HCC tissues was evaluated by qRT-PCR. F ATG5 expression was positively correlated with TINCR expression (r = 0.76, p < 0.0001). G When LCSCs were treated with Baf-A1 (75 nM) or ATG5 siRNA, sphere formation was attenuated both in these two groups. Data are shown as means ± SD. *p < 0.05.

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