Fig. 1: NaIO3-induced degenerative retinae underwent inflammatory responses and displayed decreased IL-4 expression. | Cell Death & Disease

Fig. 1: NaIO3-induced degenerative retinae underwent inflammatory responses and displayed decreased IL-4 expression.

From: IL-4 induces reparative phenotype of RPE cells and protects against retinal neurodegeneration via Nrf2 activation

Fig. 1

A, B GO enrichment of the upregulated DEGseq in NaIO3-induced degenerative retinae showed the enriched differentially expressed mRNAs in biological processes (A) and molecular function (B). C qPCR analysis showed decreased mRNA levels of IL-4 in NaIO3-induced degenerative retina, while the pro-inflammatory cytokines and chemokines/chemokine receptors, including Ifn-γ, Il-6, Tnf-α, Cxcl1, Cxcl2, Cxcl10, Cxcr1 and Cxcr2 were significantly increased. The anti-inflammatory Il-10 had no significant difference between the NaIO3-induced retina and normal control, n = 3. D Western blotting analysis showed marked decreased IL-4 expression in the NaIO3-retinae in comparison to controls, n = 3. E Lack of IL-4 expression (red) and broken ZO-1-positive RPE barrier (green) were observed in the NaIO3-induced RPE flat-mount sheet by immunohistochemistry analysis. Scale bar, 50 µm. n = 3. Data are shown as mean ± SEM, **P < 0.01, ***P < 0.001, n.s: not significant, unpaired Student’s t-test in C and D.

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