Fig. 6: IL-4 induced antioxidant and anti-inflammatory genes expression in vivo. | Cell Death & Disease

Fig. 6: IL-4 induced antioxidant and anti-inflammatory genes expression in vivo.

From: IL-4 induces reparative phenotype of RPE cells and protects against retinal neurodegeneration via Nrf2 activation

Fig. 6

A Immunoflorence of cryosection showed high expression of Nos2 in response to NaIO3 in the ZO-1+ RPE monolayer, whereas IL-4 treatment decreased Nos2 and increased Arg1 expression. Scale bar: 20 μm. B In the RPE flat-mounts, NaIO3 disrupted the well-organized hexagonal-shaped RPE cell and induced Nos2 expression elevation, whereas IL-4 treatment reversed it with more organized barrier structures of RPE cells. IL-4 could also suppress the Nos2 expression and promote Arg1 expression within RPE cells. Scale bar: 20 μm. C qPCR data showed the reduction of Nos2 and increase of Arg1 in the NaIO3 + IL-4 group. In addition, IL-4 treatment induced the expressions of anti-oxidative stress-related genes, including Hmox1, Nqo1, Foxm1, and Gpx1 in the NaIO3-retina-RPE complex. n = 3. D Luminex results showed elevated expression of pro-inflammatory cytokines in the RPE complex of NaIO3-induced model, including G-Csf, Cxcl1, and Ccl2, which decreased after IL-4 treatment. n = 3. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s: no significance, one-way ANOVA test.

Back to article page