Fig. 5: LINC00924 regulates the P38/PPARĪ± signaling pathway via hnRNPC regulated Mnk2 pre-mRNA AS. | Cell Death & Disease

Fig. 5: LINC00924 regulates the P38/PPARĪ± signaling pathway via hnRNPC regulated Mnk2 pre-mRNA AS.

From: LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

Fig. 5

A Heatmap presenting significantly differentially expressed transcripts between MGC803-NC and LINC00924-OE cell lines. B A schematic model of Mnk2 AS. MNK2 pre-mRNA can be alternatively spliced to yield two isoforms: Mnk2a and Mnk2b. Mnk2a contains e14a, a binding site for MAP kinases, while Mnk2b does not [30]. C The correlations of LINC00924 expression with Mnk2b (B) and Mnk2a (C) expression in GC tissues. D RTā€“PCR analysis of Mnk2a and Mnk2b in MGC803-NC and LINC-OE cell lines and in AGS-NC and LINC-KD cell lines. E Schematic illustration of base pairing between LINC00924 and the e14a of Mnk2. F qRTā€“PCR detection of e14a enrichment by LINC00924 using a RAP assay. G RTā€“PCR analysis of MGC803-NC and LINC-OE cell lines treated with si-hnRNPC or NC. H hnRNPC knockdown did not disrupt the RNAā€“RNA duplex formed by LINC00924 and e14a. I RIP analysis conducted using an anti-hnRNPC antibody to validate the interaction between hnRNPC and the e14a of Mnk2. J LINC00924 is essential for the formation of the RNAā€“RNA duplex by LINC00924 and e14a.

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