Fig. 3: DKK1 is highly expressed in GBM and promotes the proliferation of glioblastoma cell lines. | Cell Death & Disease

Fig. 3: DKK1 is highly expressed in GBM and promotes the proliferation of glioblastoma cell lines.

From: SMARCC2 mediates the regulation of DKK1 by the transcription factor EGR1 through chromatin remodeling to reduce the proliferative capacity of glioblastoma

Fig. 3

A Relative DKK1 expression in patient samples stratified according to the Verhaak classification based on the TCGA database. B Overall survival analysis of patients with high (red) versus low (blue) DKK1 mRNA expression based on the TCGA database-GBM and TCGA database-LGG. C Western blotting of DKK1 indicated high expression in U87MG, A172, but low expression in T98G, U118MG, LN229, and U251 cells. D The protein expression level of DKK1 was detected by western blot analysis in U87MG stably overexpressing SMARCC2 and U118MG cell line with a specific knockout of SMARCC2, respectively. E The mRNA expression level of DKK1 was detected by western blot analysis in U87MG, stably overexpressing SMARCC2 and U118MG cell line with a specific knockout of SMARCC2, respectively. F Changing the expression of DKK1 found that the protein expression of SMARCC2 did not change. G Changes in cell proliferation ability after overexpression and knockdown of DKK1 in U87MG cells and U118MG cells, respectively, detected by EDU staining. H Cell proliferation in siDKK1 U87MG cell or oeDKK1 U118MG cell were analyzed by CCK-8 assay at 24, 48, 72, 96, and 120 h after transfection. Data are expressed as mean ± SEM. ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001.

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