Fig. 4: Wdr4 deficiency in cerebellar GNPs leads to IGL and ML size reduction and Purkinje neuron disorganization.
From: Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation
A–F Representative confocal images (A) and quantitative data (B–F) showing the sizes of cerebellum (A, B), IGL (A, C), ML (A, D), and the number (A, E) as well as the density (A, F) of Purkinje neurons, in the P30 Wdr4 A-cKO and control mice. The upper-left panel in (A) was stitched with several images to display the whole cerebellum. The lower panels in (A) are higher magnification images of the boxed regions in the upper panels, showing disorganized Purkinje neurons in P30 Wdr4 A-cKO cerebella (scale bars, 500 μm in the upper panels and 50 μm in the lower panels). Data were from 3 cerebella in each group and analyzed using two-tailed unpaired Student’s t-test without Welch’s correction (equal variances), p = 0.0039 in (B), < 0.0001 in (C), 0.0027 in (D), 0.4276 in (E), and 0.0002 in (F). Data are represented as individual points and mean; **p < 0.005, ***p < 0.0005; n.s., non-significant.
