Fig. 5: Wdr4 ablation in cerebellar GNPs impairs locomotion and coordination.
From: Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation
A–C Representative moving traces (A) and quantitative data (B, C) for the moving distance (B) and time spent in the inner zone (C) in an open field test for Wdr4 A-cKO and control mice. D Quantitative data for latency to fall in rotarod test for Wdr4 A-cKO and control mice. E Quantitative data for grip strength in Wdr4 A-cKO and control mice. F Quantitative data for foot faults in grid-walking test for Wdr4 A-cKO and control mice. G–Q Quantitative data for walking speed (G), step length (K), stride length (L), stance phase time (I), double support time (J), base of support (M), foot angle (N), print length (O), intermediary toe spread (P), and toe spread (Q) in gait analyses for Wdr4 A-cKO and control mice. R Schematic representation of the parameters measured in gait analysis. Data were from 10 adult male mice (2–3 months old) in each group. Data following a normal distribution were analyzed using two-tailed unpaired Student’s t-test with Welch’s correction (unequal variances, I, J, M, O) or without Welch’s correction (equal variances, B–G, K, L, N, P), depending on the F-test results. Data not following a normal distribution were analyzed using Mann–Whitney test (H, Q). p = 0.0058 in (B), 0.0215 in (C), 0.0232 in (D), 0.2493 in (E), <0.0001 in (F), < 0.0001 in (G), 0.0605 in (H), < 0.0001 in (I), < 0.0001 in (J), 0.0008 in (K), 0.0007 in (L), 0.0003 in (M), 0.0018 in (N), 0.0005 in (O), 0.0003 in (P), and 0.0007 in (Q). Data are represented as individual points and mean ± S.E.M.; *p < 0.05, **p < 0.005, ***p < 0.0005; n.s., non-significant. See also Videos S2A and S2B.
