Fig. 2: MSCs-IT treatment reduced neutrophil infiltration in psoriatic lesions. | Cell Death & Disease

Fig. 2: MSCs-IT treatment reduced neutrophil infiltration in psoriatic lesions.

From: Mesenchymal stem/stromal cells primed by inflammatory cytokines alleviate psoriasis-like inflammation via the TSG-6-neutrophil axis

Fig. 2

Mice were randomly divided into four groups: the vehicle mice, the PBS-treated psoriatic mice, the MSC-treated psoriatic mice, and the MSC-IT-treated psoriatic mice. The mice were euthanized on day 7. The skin and spleen were analyzed by H&E staining, IHC, and flow cytometry. A, B Representative images of neutrophils analyzed by flow cytometry of skin and spleen. All frequencies referred to viable cells. Open and filled histogram represented neutrophils in the indicated groups. C H&E staining of lesions in the indicated groups. Red arrows highlighted neutrophils. D The Ly6G positive cells (neutrophils) of skin were visualized by IHC. Scale bars: 50 µm. Moreover, the specimens of patients were used for H&E staining and IHC analysis. E H&E staining of skin in the indicated groups. F IHC analysis of neutrophil infiltration in the lesions. Scale bars: 75 µm. G Statistics of CD15 positive cells. Data were shown as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant.

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