Fig. 1: PNO1 is a negative regulator of ferroptosis in HCC. | Cell Death & Disease

Fig. 1: PNO1 is a negative regulator of ferroptosis in HCC.

From: PNO1 inhibits autophagy-mediated ferroptosis by GSH metabolic reprogramming in hepatocellular carcinoma

Fig. 1

A The proteomic analysis of Hep3B sh-Ctrl and sh-PNO1 cells. B Western Blotting analysis of PNO1 protein level in Hep3B sh-PNO1 and HLE PNO1 cells compared with their parental cells. C, D The mRNA expression of PNO1 in Hep3B sh-PNO1 (C) and HLE PNO1 (D) cells was detected with qRT-PCR (*P < 0.05, **P < 0.01). E, F The colony formation assay shown the growth effect of PNO1 in Hep3B sh-PNO1 (E) and HLE PNO1 cells (F) compared with their parental cells (***P < 0.001). G Representative transmission electron microscopy images of Hep3B sh-Ctrl and sh-PNO1 cells. H, I The ROS levels in indicated Hep3B (H) and HLE cells were assayed by DCFH-DA staining (I) (**P < 0.01, *P < 0.05). J, K The lipid ROS levels in indicated Hep3B (J) and HLE cells were measured by BODY staining (K) (***P < 0.001).

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