Fig. 4: PNO1 regulates the expression of SLC7A11 via p53. | Cell Death & Disease

Fig. 4: PNO1 regulates the expression of SLC7A11 via p53.

From: PNO1 inhibits autophagy-mediated ferroptosis by GSH metabolic reprogramming in hepatocellular carcinoma

Fig. 4

A Cluster analysis of the correlation between PNO1 expression and ferroptosis-related proteins. B Western Blotting analysis of ferroptosis-related protein levels in indicated Hep3B and HLE cells. C, D The mRNA expression of ferroptosis-related genes was shown in indicated Hep3B (C) and HLE (D) cells (*P < 0.05, **P < 0.01, ***P < 0.001). E The relative GSH levels of Hep3B sh-Ctrl and sh-PNO1 cells treated with SLC7A11 recombinant protein (***P < 0.001, **P < 0.01). F The lipid ROS levels of Hep3B sh-Ctrl and sh-PNO1 cells treated with SLC7A11 recombinant protein (*P < 0.05, **P < 0.01). G Heatmap showing the correlation between expression of PNO1 and p53 pathway-related proteins. H GSEA showing the correlation between PNO1 expression and the p53 pathway genes. I The protein levels of SLC7A11 transcriptional regulators in indicated Hep3B cells. J The protein levels of SLC7A11 in sh-p53 cells.

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