Fig. 6: PNO1 promotes ferroptotic cancer cell death in vivo. | Cell Death & Disease

Fig. 6: PNO1 promotes ferroptotic cancer cell death in vivo.

From: PNO1 inhibits autophagy-mediated ferroptosis by GSH metabolic reprogramming in hepatocellular carcinoma

Fig. 6

A–C Images of tumors from nude mice (A), Tumor volumes (B), and weights (C) in Hep3B sh-ctrl and sh-PNO1 groups after treatment with sorafenib (**P < 0.01, *P < 0.05). D–F Images of tumors from nude mice (D), Tumor volumes (E), and weights (F) in HLE vector and PNO1 groups (**P < 0.01, *P < 0.05). G, H Western Blotting analysis of ferroptosis-related proteins of Hep3B sh-ctrl, sh-PNO1 groups (G) and HLE vector, PNO1 groups (H) in established xenograft model assessed. I, J The mRNA expression of ferroptosis-related genes in Hep3B sh-ctrl, sh-PNO1 groups (I) and HLE vector, PNO1 groups (J) was observed (***P < 0.001, **P < 0.01, *P < 0.05). K, L The intracellular glutamate levels were measured in Hep3B sh-ctrl, sh-PNO1 groups (K) and HLE vector, PNO1 groups (L) (*P < 0.05, ***P < 0.001). M, N The cysteine levels were assayed in Hep3B sh-ctrl, sh-PNO1 groups (M) and HLE vector, PNO1 groups (N) (*P < 0.05). O, P The relative GSH levels of Hep3B sh-ctrl, sh-PNO1 groups (O) and HLE vector, PNO1 groups (P) were shown (*P < 0.05). Q, R The relative MDA levels of Hep3B sh-ctrl, sh-PNO1 groups (Q) and HLE vector, PNO1 groups (R) were detected (**P < 0.01, *P < 0.05).

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