Fig. 3: PFKP Y64 phosphorylation induces VEGF expression through HIF-1 α expression and β-catenin Ser552 phosphorylation in response to EGFR activation. | Cell Death & Disease

Fig. 3: PFKP Y64 phosphorylation induces VEGF expression through HIF-1 α expression and β-catenin Ser552 phosphorylation in response to EGFR activation.

From: Mutual regulation between phosphofructokinase 1 platelet isoform and VEGF promotes glioblastoma tumor growth

Fig. 3

WB and qRT-PCR were performed with indicated primers and antibodies, respectively (A–E). A LN229/EGFRvIII cells with or without PFKP depletion and with or without reconstituted expression of WT Flag-rPFKP or Flag-rPFKP Y64F mutant were transfected with or without Flag-HIF-1α and HA-myr-AKT. B Serum starved LN229 cells were pretreated with DMSO or HIF inhibitor (10 μM) for 1 h and then treated with EGF (100 ng/mL) for 12 h. C LN229 cells were transfected with a control vector or CA β-catenin. D LN229/EGFRvIII cells with stable expression of β-catenin shRNA or a control shRNA were reconstituted with or without WT rβ-catenin or rβ-catenin S552A mutant. E PFKP-depleted LN229/EGFRvIII cells with or without reconstituted expression of WT Flag-rPFKP or Flag-rPFKP Y64F mutant were transfected with or without CA β-catenin and Flag- HIF-1α. Data are presented as mean ± standard deviation of three independent experiments (A–E). **P < 0.01; ***P < 0.001, based on the Student’s t-test or one-way ANOVA with Tukey’s post hoc test.

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