Fig. 4: PFKP Y64 phosphorylation induces HIF-1 α expression, β-catenin S552 phosphorylation, and VEGF expression and promotes blood vessel formation in vivo. | Cell Death & Disease

Fig. 4: PFKP Y64 phosphorylation induces HIF-1 α expression, β-catenin S552 phosphorylation, and VEGF expression and promotes blood vessel formation in vivo.

From: Mutual regulation between phosphofructokinase 1 platelet isoform and VEGF promotes glioblastoma tumor growth

Fig. 4

A Representative H&E staining images of intracranial xenografts bearing PFKP-depleted LN229/EGFRvIII cells with or without reconstituted expression of WT Flag-rPFKP or Flag-rPFKP Y64F mutant with or without myr-AKT1 (A; upper panel) and quantification of tumor volumes (A; bottom panel). Scale bar, 2 mm. B IHC analyses of tumor tissues with the indicated antibodies (upper panel). Quantification of indicated IHC staining (bottom panel). Scale bar, 100 μm. C, E IHC staining of human GBM specimens was performed with indicated antibodies (n = 25). Representative images from the staining of three different specimens are shown. Scale bar, 100 μm. D, F The IHC stains were scored, and correlation analyses were performed. The Pearson correlation test was used. Note that the scores of some samples overlapped. ***P < 0.001, based on one-way ANOVA with Tukey’s post hoc test.

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