Fig. 4: circFNDC3B increases RNF41 mRNA stability and expression via binding to FXR2. | Cell Death & Disease

Fig. 4: circFNDC3B increases RNF41 mRNA stability and expression via binding to FXR2.

From: m6A-modified circFNDC3B inhibits colorectal cancer stemness and metastasis via RNF41-dependent ASB6 degradation

Fig. 4

A The RNF41 level in CRC tissues was detected by qRT-PCR. B The correlation between circFNDC3B and RNF41 level in CRC tissues was analyzed by spearman correlation analysis. C The interaction between biotinylated circFNDC3B and FXR2 was detected by RNA pull-down assay. Random probe acted as a negative control. D The interaction between circFNDC3B and FXR2 was detected by RIP assay. Normal IgG served as a negative control. E The co-localization of circFNDC3B and FXR2 was detected by RNA FISH and immunofluorescence (IF). Scale bar, 10 μm; Green, circFNDC3B; Red, FXR2; Blue, DAPI. F, G The interaction between RNF41 and FXR2 was detected by RIP assay. Normal IgG served as a negative control. H The mRNA stability of RNF41 was determined by RNA stability assay. I, J The mRNA and protein levels of RNF41 in transfected CRC cells were detected by qRT-PCR and Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001.

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