Fig. 8: Overexpression of ASB6 reverses circFNDC3B or RNF41-mediated regulation of CRC stemness and metastasis.

A–E The expression of stemness markers were detected by qRT-PCR and western blotting. F Representative sphere images of transfected CRC cells with quantitative analysis. G CD133+ cells were analyzed by flow cytometry with quantitative analysis in transfected CRC cells. H Cell migration was monitored by wound healing assay with quantitative analysis in transfected CRC cells. I Cell invasion was measured by Transwell invasion assay with quantitative analysis. *P < 0.05, **P < 0.01.