Table 1 Primers used for qRT-PCR analysis.

From: m6A-modified circFNDC3B inhibits colorectal cancer stemness and metastasis via RNF41-dependent ASB6 degradation

Genes

Primer sequences (5′-3′)

circFNDC3B

F: 5′-GCAAGAAGCAGCCCAAAGTC-3′

 

R: 5′-CATGGCTGAGGGGTAGCTTG-3′

OCT4

F: 5′-CTTGAATCCCGAATGGAAAGGG-3′

 

R: 5′-GTGTATATCCCAGGGTGATCCTC-3′

Nanog

F: 5′-GTCCCAAAGGCAAACAACCC-3′

 

R: 5′-ATCCCTGCGTCACACCATTG-3′

SOX2

F: 5′-GCCCTGCAGTACAACTCCAT-3′

 

R: 5′-GACTTGACCACCGAACCCAT-3′

CD133

F: 5′-ATGCTCTCAGCTCTCCCGC-3′

 

R: 5′-TTCTGTCTGAGGCTGGCTTG-3′

RNF41

F: 5′-AAAATGTGAGAAGGGAGCAGCA-3′

 

R: 5′-CACAGTCGCTGAGGTGAGAC-3′

ASB6

F: 5′-GGAAAGCCCACTCTCCCTTTT-3′

 

R: 5′-CTTGACGTCTGCCCCATGCT-3′

GAPDH

F: 5′-CCAGGTGGTCTCCTCTGA-3′

 

R: 5′-GCTGTAGCCAAATCGTTGT-3′

U6

F: 5′-CTCGCTTCGGCAGCACA-3′

 

R: 5′-AACGCTTCACGAATTTGCGT-3′

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