Fig. 6: Histologic analysis and gene expression validates improvement of steatosis and fibrosis effects of J2H-1702 in Amylin liver NASH mouse model. | Cell Death & Disease

Fig. 6: Histologic analysis and gene expression validates improvement of steatosis and fibrosis effects of J2H-1702 in Amylin liver NASH mouse model.

From: Inhibition of 11β-hydroxysteroid dehydrogenase 1 relieves fibrosis through depolarizing of hepatic stellate cell in NASH

Fig. 6

Male C57BL/6 N mice were maintained on AMLN-diet for 30 weeks and were continued on the same diet during the treatment. Reference compound (30 mg/kg Obeticholic acid, OCA) and test compounds (3, 10, 20 mg/kg J2H-1702) were treated orally for 12 weeks. A Liver tissues of all the group animals were collected and were stained with H&E, Oil Red O, Sirius RED and ER-TR7. Collagen proportion area, steatosis, liver ER-TR7 levels were measured from tissue staining. B, C Gene expression of TNFα, IFNγ, IL6 B and TIMP1, Col1α1, α-SMA C were analyzed by quantitative RT-PCR in liver tissue. Values are expressed as Mean ± SEM. ####P < 0.0001 vs Lean chow, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs AMLN diet control. One way ANOVA followed by Dunnett’s test.

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