Fig. 4: 4EBP1 plays a crucial role in cell apoptosis under glucose deprivation conditions.
From: 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer
A, B A549 and H1299 cells transfected with either control (CTL) siRNA or 4EBP1 siRNAs (A), A549 and H1299 cells were transfected with CTL siRNAs or 4EBP1 siRNA or cotransfected with CTL siRNAs and mutant plasmid eIF4E-S209E (B). Cells were treated with normal or glucose starvation. Cell growth rate was assessed by MTT assay. The data represent the averages of three independent experiments (mean ± SD). *P < 0.05, **P < 0.01, ***P < 0.001. C A549 cells transfected with either control siRNA or 4EBP1 siRNAs were treated with normal or glucose starvation for 16 h. The changes of cell morphological was investigated (scale bar = 100 µm, magnification: ×100). D A549 cells transfected with either control siRNA or 4EBP1 siRNAs were treated with normal or glucose starvation for 16 h. Cell apoptosis analysis was done by flow cytometry. E A549 cells transfected with either control siRNA or 4EBP1 siRNAs were treated with normal (0 h) or glucose starvation. The expression levels of the apoptosis related proteins were checked by western blot. C-Caspase3: Cleaved-Caspase3. F, G parental A549 (CON) and A549 stable cell lines with 4EBP1 knockdown (A549-sh4EBP1) were treated with normal or glucose starvation for 12 h. Lactic acid levels were monitored (F, upper panel). ECAR (F, lower panel) and OCR (G) measurements were done.
