Fig. 6: 4EBP1 inhibits tumor progression under glucose deprivation conditions in vivo.
From: 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer
A Western blot analysis of 4EBP1 expression in parental A549 and A549-sh4EBP1 cells. B Parental A549 and A549-sh4EBP1 cells were treated with normal or glucose starvation for 48 h. Cell growth rate was assessed by MTT assay. The data represent the averages of three independent experiments (mean ± SD). *P < 0.05. C Parental A549 and A549-sh4EBP1 cells were treated with normal or glucose starvation for 16 h. The changes of cell morphological was investigated (scale bar = 100 µm, magnification: ×100). D Parental A549 and A549-sh4EBP1 cells were treated with normal or glucose starvation for 20 h. Cell apoptosis analysis was done by flow cytometry. E, F In vivo xenograft assay was performed using parental A549 and A549-sh4EBP1 cells, tumors were photographed (E), and their weights and volumes were measured (F). The P value was calculated by paired t-test. *P < 0.05. G Immunohistochemical staining in the tumor-central region of tumor induced by parental A549 and A549-sh4EBP1 cells for 4EBP1, p-STAT3, BCL2, MCL1, Survivin and Cleaved PARP1 (C-PARP1). Scale bars: 50 μm, magnification: ×200.
