Fig. 2: BMP4 inhibits progenitor Leydig cell (PLC) differentiation in vitro. | Cell Death & Disease

Fig. 2: BMP4 inhibits progenitor Leydig cell (PLC) differentiation in vitro.

From: Bone morphogenetic protein 4 inhibits rat stem/progenitor Leydig cell development and regeneration via SMAD-dependent and SMAD-independent signaling

Fig. 2

A PLCs were incubated with BMP4 for 24 h. B–D Androsterone (AO), T, AO + T output after BMP4 treatment; Mean ± SEM, n = 4. E QPCR detection of Bmpr1a, Lhcgr, Star, Cyp11a1, Hsd3b1, and Akr1c14 expression which was normalized to Rps16 (an internal control); Mean ± SEM, n = 4. F Western blot. G Quantitative results of BMPR1A, LHCGR, STAR, CYP11A1, HSD3B1, and AKR1C14 protein levels which were normalized to ACTB (an internal control); Mean ± SEM, n = 3. *P < 0.05, **P < 0.01 and ***P < 0.001 indicate significant differences compared to control (0 ng/mL BMP4).

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