Fig. 4: The effect of BMP4 on stem Leydig cell (SLC) proliferation in vitro. | Cell Death & Disease

Fig. 4: The effect of BMP4 on stem Leydig cell (SLC) proliferation in vitro.

From: Bone morphogenetic protein 4 inhibits rat stem/progenitor Leydig cell development and regeneration via SMAD-dependent and SMAD-independent signaling

Fig. 4

A–F CD90+ SLCs were incubated with 0 (A), 0.01 (B), 0.1 (C), 1.0 (D), and 10.0 (E) ng/mL BMP4 for 24 h. F Quantitative data for EdU labeling index, mean ± SEM, n = 6. White arrowheads indicate EdU+ SLCs: CD90, red membrane; EdU, green nucleus; DAPI, blue nucleus. Bar = 50 μm. SLCs on ST seminiferous tubule (ST) surface treated with 0 (G), 0.01 (H), 0.1 (I), 1.0 (J), and 10.0 (K) ng/mL of BMP4 for 7 d and then EdU (green) was incorporated; L Quantitative results for EdU+ SLCs per square centimeter, mean ± SEM, n = 6. White arrows indicate EdU+ SLCs. Bar = 50 μm. M ST culture protocol; N Medium testosterone (T) levels after BMP4 treatment in the first week; Mean ± SEM, n = 6. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences compared to control (0 ng/mL BMP4).

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