Fig. 2: Atg7 knockdown prevents p21 suppression-dependent increased LC3 turnover in autophagy-competent cells. | Cell Death & Disease

Fig. 2: Atg7 knockdown prevents p21 suppression-dependent increased LC3 turnover in autophagy-competent cells.

From: Inhibition of p21 activates Akt kinase to trigger ROS-induced autophagy and impacts on tumor growth rate

Fig. 2

A Isogenic p21+/+ and p21−/− HCT116 cells were stably transduced with scrambled or Atg7 targeting shRNA and analyzed by western blotting for LC3 conversion. B, C HCT116 p21wt cells, with stable expression of control and Atg7 shRNA, were subjected to p21 inhibition either by RNA silencing (B) or UC2288 (C) followed by immunoblot detection of LC3-I and II. D Western blot analysis of LC3-I/II in three biological replicates of DU145 cell lysates after transduction with control or p21 shRNA.

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