Fig. 4: p53 independent autophagy modulation by p21. | Cell Death & Disease

Fig. 4: p53 independent autophagy modulation by p21.

From: Inhibition of p21 activates Akt kinase to trigger ROS-induced autophagy and impacts on tumor growth rate

Fig. 4

HCT116 p53wt and null cells were subjected to p21 attenuation either by sh-p21 lentivirus transduction (A) or by treating with UC2288 (B). Cells were then harvested and immunoblotting was carried out to determine relative expression of p53, p21, and LC3. Actin was probed as a normalization control. Densitometric analysis for LC3-II expression of three independent experiments was performed by ImageJ and represented graphically as mean ± SE. *P < 0.05. **P < 0.01.

Back to article page