Fig. 6: p21 modulated autophagy affects in vivo tumor growth kinetics. | Cell Death & Disease

Fig. 6: p21 modulated autophagy affects in vivo tumor growth kinetics.

From: Inhibition of p21 activates Akt kinase to trigger ROS-induced autophagy and impacts on tumor growth rate

Fig. 6

A Approximately 500 numbers of HCT116 p21+/+ and p21−/− cells per well were grown in a six-well plate for 2 weeks, thereafter fixed and stained with crystal violet. B Quantitative analyses of colony area by ImageJ. (mean ± SE; ***P < 0.001) C, D NOD/SCID mice were subcutaneously injected individually with equal numbers (5 × 106) of HCT116 p21wt and null cells at the right and left flank, respectively. Tumors were allowed to grow till 3 weeks after which the mice were sacrificed to dissect the tumors (D) for weighing (C); symbols represent individual mouse; data presented as mean ± SE. E Tumor size were recorded at regular intervals during the study and their volumes were calculated as described in Methods section. (n = 7 per group; mean ± SE are shown). F Tumor tissue lysates were analyzed by immunoblotting to confirm p21 expression in respective xenografts and determine relative conversion of LC3.

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